EGFR protein overexpression and gene amplification in squamous cell carcinomas of the esophagus.

摘要: Overexpression of epidermal growth factor receptor (EGFR) is observed in many cancers, sometimes accompanied by gene amplification. Recently, several clinical therapies targeting EGFR were developed, but the eligibility criteria for these not fully established. To develop such esophageal squamous cell carcinoma (ESCC), we sought to clarify: (i) exact frequency overexpression, (ii) relationship between protein overexpression and amplification, (iii) amplification specific mutations (iv) correlation status or pathological features. Immunohistochemistry revealed that overexpressed 53 (50%) 106 ESCC examined. Fluorescence situ hybridization (FISH) indicated clear 15 tumors, somewhat higher copy 32 cases, no increase 6 cases. Gene was significantly associated with high level overexpression. Direct sequencing exons 19 21 tumors exhibiting 25 correlated depth invasion tumor. In conclusion, anti-EGFR may be appropriate patients ESCC. We assume combined analyses immunohistochemistry/FISH would clarify aberrations function, could help identify those who benefit from therapy.