Involvement of the MAP kinase cascade in Xenopus mesoderm induction.

摘要: Mitogen-activated protein kinase (MAPK) is activated by MAPK (MAPKK) in a variety of signaling pathways. This cascade has been shown to function cell proliferation and differentiation, but its role early vertebrate development remains be investigated. During embryogenesis, the induction patterning mesoderm are thought determined signals from intercellular factors such as members fibroblast growth factor (FGF) family transforming factor-beta family. Here we show that microinjection either mRNA encoding constitutively active mutant MAPKK or form STE11, kinase, leads ectodermal explants Xenopus embryos. Moreover, expression phosphatase-1 (MKP-1, also called CL100) blocks factor-stimulated induction. Furthermore, injection CL100 into two-cell stage embryos causes severe defects gastrulation posterior development. The effects induced can rescued co-injection wild-type mRNA. Thus, may play crucial especially during