Molecular assessment of depth of myometrial invasion in stage I endometrial cancer: a model based on K-ras mutation analysis.

摘要: Abstract Introduction Overall nearly 20% of endometrial cancer (EC) patients die the disease and over half these had initially presented with clinical stage I disease. There is a strong correlation between mortality depth myometrial invasion. Current assessment invasion relies on light microscopy. Tumor cells can evade detection by microscopy if they are vastly outnumbered cells. Molecular techniques have great potential in such isolated Objective The objective was to develop model for application molecular advance risk status EC. Methods study sample included 21 ECs documented K-ras mutation from two series 96 106 United Kingdom Norway, respectively. using heteroduplex mobility analysis amplified created restriction site, followed sequencing identify specific base substitution at codon 12 13 oncogene. For each case mutation, modified mutant allele-specific amplification technique carried out tissue strips microdissected increasing depths myometrium underlying tumor. been previously examined histologically absence infiltrating tumor Presence mutations used within normal myometrium. Correlations submicroscopic cell infiltration clinicopathological factors were studied. Results Of cases 6 (28%) showed evidence beyond histological boundary. found be variable. staging tumors would changed 3 (14%) detected histologically. A borderline significant presence noted ( P = 0.053). recurrence rate survival without better than those with, although it did not reach statistical significance (recurrence 0.13, free 0.14, cause-specific 0.12, total 0.2). Conclusions feasible may add information conventional Further prospective studies required define this technology.