Onchocerca gutturosa and O. volvulus: studies on the viability and drug responses of cryopreserved adult worms in vitro

摘要: Abstract The viability and drug responses of cryopreserved adult Onchocerca have been examined in vitro. Male worms were liquid nitrogen (−196 °C) using ethanediol as a cryoprptectant 2-step incubation procedure. After thawing, 85–90% O. gutturosa males normally motile. These motile evaluated for 4 measurements (long-term motility/survival culture; [U-14C]adenine uptake leakage; glucose utilization; MTT-formazan colorimetry) no different from unfrozen controls. Subsequent experiments demonstrated that the motility exposed to antifilarial drugs ivermectin, CGP 6140 levamisole virtually identical Some success was also obtained with this technique cryopreserving volvulus males, 2 thawed specimens surviving culture 93 106 d respectively. Following collagenase isolation, female medium +10% serum without protectant at −79 °C. A batch 8 all when 14 later, mean survival time (based on 5 specimens) 71 (range 60–90). However, transferred °C −196 badly damaged thawed. Female Guatemala sent by air freight solid CO2 UK. Most active Survival ranged 7 272 culture. It is concluded these techniques are practical value storage transportation Onchocerca.