Drug activity against Onchocerca gutturosa males in vitro: a model for chemotherapeutic research on onchocerciasis.

摘要: An in vitro system for chemotherapeutic research using adult male Onchocerca gutturosa has been developed as a model O. volvulus. Using culture consisting of medium MEM + 10% heat inactivated foetal calf serum (IFCS) LLCMK2 (monkey kidney) feeder cells an atmosphere 5% CO2 air, we examined the effects range antiparasitic drugs on worm motility. Ivermectin, levamisole, furapyrimidone, Mel W, chloroquine, metrifonate, flubendazole, amoscanate and Ciba-Geigy compounds CGP 6140, 20'376 CGI 17658 either immobilized or significantly reduced motility levels at concentration 5 X 10(-5) M less within 7-day period. Worms were affected very low concentrations by ivermectin (effective conc. to reduce 50% controls, 3.14 10(-8) M), levamisole (7.95 6140 (8.87 10(-9) M) (2.78 M). Difficulties experienced accurately repeating immotile endpoint due inconsistent partial recovery Over period diethylcarbamazine had little effect levels, while suramin caused slight increase activity compared controls some timepoints. Subsequent experiments demonstrated differences drug efficacy depending presence absence probably because avidly binding proteins. However, found be essential ingredients maintain worms good condition, indicating that new should evaluated both cells. Comparisons made between responses Brugia pahangi certain these species differ their sensitivities novel compound (Wellcome), parasites used wherever possible identification development intended treatment onchocerciasis. The described here, gutturosa, provides basis further practical alternative