‘Video-scats’: combining camera trapping and non-invasive genotyping to assess individual identity and hybrid status in gray wolf

作者: Antonio Canu , Luca Mattioli , Alberto Santini , Marco Apollonio , Massimo Scandura

DOI: 10.2981/WLB.00355

关键词: Population sizeSampling (statistics)BiologyPopulationMicrosatelliteItalian wolfIntrogressionGray wolfCartographyGenotypingGenetics

摘要: Motion-activated video cameras and non-invasive genetic sampling are tools commonly used to obtain relevant information on wild populations of rare or elusive carnivores while minimizing disturbance. The two approaches usually implemented separately, but they occasionally integrated at a population level, mostly in order estimate size. Here we show the advantages combining camera trapping genotyping an individual monitored Italian wolf affected by introgression from domestic dogs. After 24 defecation events recorded traps located marking sites, samples (‘video-scats’) were collected determine individuals' identity based analysis sex markers, 11 autosomal microsatellites, Y-chromosome microsatellites control region mitochondrial DNA. Genetic data for 19 successfully genotyped scat combined with morphological behavioural traits observed videos compared ...

参考文章(45)
Bernardino Ragni, Stefano Anile, Maria Vittoria Mazzamuto, Carmelo Arrabito, Davide Scornavacca, A non-invasive monitoring on European wildcat ( Felis silvestris silvestris Schreber, 1777) in Sicily using hair trapping and camera trapping: does it work? Hystrix-italian Journal of Mammalogy. ,vol. 23, pp. 45- 50 ,(2012) , 10.4404/HYSTRIX-23.2-4657
Edoardo Velli, Marco A. Bologna, Castelli Silvia, Bernardino Ragni, Ettore Randi, Non-invasive monitoring of the European wildcat ( Felis silvestris silvestris Schreber, 1777): comparative analysis of three different monitoring techniques and evaluation of their integration European Journal of Wildlife Research. ,vol. 61, pp. 657- 668 ,(2015) , 10.1007/S10344-015-0936-2
Ettore RANDI, Genetics and conservation of wolves Canis lupus in Europe Mammal Review. ,vol. 41, pp. 99- 111 ,(2011) , 10.1111/J.1365-2907.2010.00176.X
Rita Lorenzini, Rita Fanelli, Goffredo Grifoni, Francesco Scholl, Rosario Fico, Wolf–dog crossbreeding: “Smelling” a hybrid may not be easy Mammalian Biology. ,vol. 79, pp. 149- 156 ,(2014) , 10.1016/J.MAMBIO.2013.07.080
D. PASSILONGO, A. BUCCIANTI, F. DESSI-FULGHERI, A. GAZZOLA, M. ZACCARONI, M. APOLLONIO, THE ACOUSTIC STRUCTURE OF WOLF HOWLS IN SOME EASTERN TUSCANY (CENTRAL ITALY) FREE RANGING PACKS Bioacoustics-the International Journal of Animal Sound and Its Recording. ,vol. 19, pp. 159- 175 ,(2010) , 10.1080/09524622.2010.9753622
Maris Hindrikson, Peep Männil, Janis Ozolins, Andrzej Krzywinski, Urmas Saarma, Bucking the Trend in Wolf-Dog Hybridization: First Evidence from Europe of Hybridization between Female Dogs and Male Wolves PLoS ONE. ,vol. 7, pp. e46465- ,(2012) , 10.1371/JOURNAL.PONE.0046465
Ettore Randi, Pavel Hulva, Elena Fabbri, Marco Galaverni, Ana Galov, Josip Kusak, Daniele Bigi, Barbora Černá Bolfíková, Milena Smetanová, Romolo Caniglia, Multilocus Detection of Wolf x Dog Hybridization in Italy, and Guidelines for Marker Selection PLoS ONE. ,vol. 9, pp. e86409- 13 ,(2014) , 10.1371/JOURNAL.PONE.0086409
Andre E. Moura, Elena Tsingarska, Michał J. Dąbrowski, Sylwia D. Czarnomska, Bogumiła Jędrzejewska, Małgorzata Pilot, Unregulated hunting and genetic recovery from a severe population decline: the cautionary case of Bulgarian wolves Conservation Genetics. ,vol. 15, pp. 405- 417 ,(2014) , 10.1007/S10592-013-0547-Y