Clone heterogeneity in diploid and aneuploid breast carcinomas as detected by FISH

摘要: We investigated the relationship between DNA ploidy and alterations in chromosomes 1, 8, 12, 16, 17, 18 63 breast carcinoma samples by static cytofluorometry fluorescence situ hybridization. Thirty specimens were diploid 33 aneuploid. In aneuploid samples, index value ranged from 1.3 to 3.1, with a main peak near tetraploid values. Diploid clones present 21 of specimens. Fluorescence hybridization analysis showed heterogeneous degree specimens: one sample was normal, 16 had three chromosome involving mostly 13 an even higher alterations. The very high number signals (four, five, or more). All affected 23 Alterations 1 17 detected similar percentage whereas monosomy more frequent samples. Overrepresentation significantly than Based on these results, we suggest that carcinomas are genetically related. Chromosome early changes. Allelic chromosomal accumulations occur during progression different mechanisms. clone heterogeneity found could not be completely explained endoreduplication led suggestion instability concurs aneuploidy development. This evolutionary pathway might clinically relevant because cause metastasis development resistance therapy. Cytometry (Comm. Clin. Cytometry) 46:50 ‐56, 2001. © 2001 Wiley-Liss, Inc.