Potassium channels and human corporeal smooth muscle cell tone: diabetes and relaxation of human corpus cavernosum smooth muscle by adenosine triphosphate sensitive potassium channel openers.

摘要: Purpose: Sustained contraction of human corporeal smooth muscle depends on continuous transmembrane calcium flux through voltage gated channels. K channels modulate membrane potential and, thus, ultimately affect flux. Therefore, we characterized relaxation responses elicited by the channel modulators pinacidil and levcromakalim isolated tissue strips. We also evaluated possibility that there may be alterations in adenosine triphosphate sensitive pharmacology/function related to presence diabetes mellitus. Materials Methods: A total 215 strips obtained from 57 male patients with organic erectile dysfunction were investigated. Cumulative concentrationresponse curves constructed at half log increments for steady state equivalently phenylephrine pre-contracted (to approximately 75% maximum) Potassium currents measured using cell attached whole patch clamp technique freshly cells. Results: concentration dependent, glibenclamide response was observed levcromakalim. Consistent such observations, electrophysiological recordings myocytes revealed (10 M.) induced potassium blocked M.). In addition, statistical analysis without more both compounds than excised those diabetes. Furthermore, not affected charybdotoxin or 4-aminopyridine but completely reversed KCl tetraethylammonium chloride. Conclusions: These data indicate subtype is likely have an important role moreover, they are molecular target modulators/openers pinacidil. Such observations consistent supposition structure/function/activity these underlie least some aspects differences sensitivity modulators.