Dietary Supplementation of High Levels of Saturated and Monounsaturated Fatty Acids to Ewes during Late Gestation Reduces Thermogenesis in Newborn Lambs by Depressing Fatty Acid Oxidation in Perirenal Brown Adipose Tissue
作者： Ching Yi Chen , Gordon E. Carstens , Corey D. Gilbert , Casey M. Theis , Shawn L. Archibeque
DOI: 10.1093/JN/137.1.43
关键词:
摘要: We hypothesized that dietary supplementation of (n-6) plus (n-3) PUFA during late gestation would increase uncoupling protein-1 (UCP1) gene expression and thereby thermogenic capacity newborn lambs. Thirty twin-bearing ewes were fed rumen-protected fat (2, 4, or 8%) high in saturated monounsaturated fatty acids (SMFA) PUFA. Lambs (n = 7-10 per ewe treatment group) placed a cold chamber at 0 degrees C for 2 h. Rectal temperature was higher birth increased more with exposure lambs from 4% supplemental than 8% SMFA (fat type x level interaction, P 0.001). Cytochrome c oxidase activity greatest brown adipose tissue (BAT) 2% 0.01). BAT had nearly 7-fold (P 0.05) UCP1 mRNA decreased by over 80% 24 h age. Supplementation tended to depress palmitate esterification into lipids 0.07) oxidation 0.003) lamb vitro, especially those SMFA. Thus, supplementing the diets depressed tolerance lambs, which reflected their ability oxidize vitro.
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