Adipogenic gene expression and fatty acid composition in subcutaneous adipose tissue depots of Angus steers between 9 and 16 months of age.

摘要: We have demonstrated that among carcass adipose tissue depots, brisket subcutaneous contains the greatest concentration of MUFA and lowest SFA. Therefore, we hypothesized depots would exhibit greater adipogenic gene expres- sion over time than other major depots. Four Angus steers, each at 9, 12, 14, 16 mo age, were harvested fresh samples collected from brisket, chuck, rib, loin, sirloin, round, flank, plate. Relative expression for C/EBPβ, PPARγ, carni- tine palmitoyltransferase-1 beta (CPT-1β), stearoyl- coenzyme A desaturase (SCD), AMP-activated protein kinase alpha (AMPKα), G-coupled recep- tor 43 (GPR43) was analyzed by quantitative real-time PCR. Expression CPT-1β 12 to 14 age (all P < 0.0001) declined very low abundance in all PPARγ (P 0.03) sirloin adi- pose tissues round tissues. The SCD did not differ 4 groups = 0.95). palmitoleic:stearic acid ratio (an estimate activity) 0.001) plate, sir- loin. Conversely, had round. In general, with high- est least SFA consistently exhibited adipo- genic expression. conclude large indices deposited before 9 during a when adipocytes highly differentiated.