Detection ofEGFRmutations andEML4-ALKrearrangements in lung adenocarcinomas using archived cytological slides

摘要: BACKGROUND Although the molecular analysis of epidermal growth factor receptor (EGFR) and anaplastic lymphoma kinase (ALK) in archived lung cancer tissues is relatively well established, genetic testing cytological material has not yet become a routine. METHODS The current study used cell samples that were obtained by bronchial brushing, transthoracic needle aspiration, or biopsy imprint preparation between 1993 2008. Islets malignant cells visually located on slides, lysed situ drop sodium dodecyl sulfate-containing buffer, subjected to standard DNA RNA extraction. Examination paraffin-embedded tissue blocks (resection specimens material) from same patients was performed parallel. RESULTS A total 75 cytological/histological adenocarcinoma sample pairs underwent polymerase chain reaction for EGFR mutation. Two 1 morphological failed produce DNA. Concordance wild-type mutation status observed 54 72 14 informative pairs, respectively; 3 pair, respectively, had only histological material. The discrepancies could be explained failure ensure high percentage analyzed or, alternatively, genuine intratumoral heterogeneity some neoplasms. extraction followed reverse transcriptase-polymerase EML4-ALK translocation 44 mutation-negative pairs; failures 2 6 specimens. All concordant either norm (32 36 pairs) presence gene fusion (4 pairs). CONCLUSIONS Archived slides appear suited both ALK analysis. Cancer (Cancer Cytopathol) 2013;121:370–376. © 2013 American Society.