Multiple microarray platforms utilized for hepatic gene expression profiling of GH transgenic coho salmon with and without ration restriction.

摘要: The objectives of this study are to examine hepatic gene expression changes caused by GH transgenesis and enhanced growth. This is the first use cDNA microarrays influence on liver in a nonmammalian vertebrate, such using sexually immature animals. Three groups coho salmon were examined: transgenic full ration (T), restricted (R), control non-transgenic (C). Specific growth rates for weight T approximately eightfold higher than C, fourfold R. Differential T, R, C samples was determined w3500 16 000 microarrays, R compared differentw4000 microarray. multiple microarray platforms increased overall proportion transcriptome considered these studies. Cross-platform comparisons identified genes behaving similarly between For example, encoding precerebellin-like protein complement component C3 downregulated relative (R!C) two studies, hemoglobins b ROC all three Comparisons informative lists within studies inferred causes altered expression. ten genes, including 78 kDa glucose-regulated protein, glycerol-3-phosphate dehydrogenase, b, C-type lectin, likely induced due their presence both TO Ca nd lists. Eleven hepcidin, nuclear p8, precerebellin-like, transketolase, fatty acid-binding present T! R!C were, therefore, suppressed transgenesis. A large number salmonid involved iron homeostasis, mitochondrial function, carbohydrate metabolism, cellular proliferation, innate immunity. Pentose phosphate pathway phosphogluconate transaldolase, dysregulated samples. Changes maintaining hemoglobin levels (heme oxygenase, hemoglobinsa andb, Kruppel-like globin activator, hepcidin) fish indicate need additional fish, perhaps metabolic rate required