Rabies DNA vaccine encoding lysosome-targeted glycoprotein supplemented with Emulsigen-D confers complete protection in preexposure and postexposure studies in BALB/c mice
作者： Manpreet Kaur , Ankur Saxena , Anant Rai , Rakesh Bhatnagar
DOI: 10.1096/FJ.09-138644
关键词:
摘要: The worldwide incidence of rabies and the inability currently used vaccination strategies to provide highly potent cost-effective therapy indicate need for an improved vaccine. Thus, DNA vaccine based on lysosome-targeted glycoprotein virus was evaluated in BALB/c mice. It imparted partial protection (60%) against challenge with 20 LD50 standard (CVS) strain virus. To improve outcome vaccination, ultimately enhance immune response, we investigated different routes delivery, varied doses DNA, influence adjuvant supplementation. highest response pertaining IgG antibody titer, a predominantly IgG1/IgG2a subclass distribution, effective cellular immunity, high level neutralizing antibodies (RVNAs) attained by optimized formulation comprising intramuscular administration 100 μg supplemented Emulsigen®-D. In preexposure p...
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