Crosstalk between EGFR and integrin affects invasion and proliferation of gastric cancer cell line, SGC7901
作者： Jian Ding , Li , Lin , Wang
DOI: 10.2147/OTT.S35322
关键词:
摘要: Background/objective To investigate the crosstalk between epidermal growth factor receptor (EGFR) and integrin-mediated signal transduction pathways in human gastric adenocarcinoma cells. Methods EGF was used as a ligand of EGFR to stimulate cell, SGC7901. Signal molecules downstream integrin, FAK(Y397) p130cas(Y410) phosphorylation, were measured by immunoprecipitation western blot. Fibronectin (Fn) integrin same cell line. extracellular signal-regulated kinase (ERK) general phosphorylation also measured. Focal adhesion (FAK) small-interfering RNA designed transfected into SGC7901 cells decrease expression FAK. Modified Boyden chambers MTT assay examine effect FAK inhibition on invasiveness proliferation Results activated while Fn ERK phosphorylation. Inhibition decreased Fn, or Fn. Conclusion There is transduction. may be key cross point two acts potential target for cancer therapy.
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