Interferon-γ Inhibits Transgene Expression Driven by SV40 or CMV Promoters but Augments Expression Driven by the Mammalian MHC I Promoter
作者： Jerome S. Harms , Gary A. Splitter
DOI: 10.1089/HUM.1995.6.10-1291
关键词:
摘要: ABSTRACT The use of mammalian gene expression vectors has become increasingly important for transgenics and therapy as well basic research. Essential the success these in medical research applications is proper choice promoter linked to interest. Many elements from pathogenic viruses, including simian virus 40 (SV40) cytomegalovirus (CMV). Lymphokines produced by immune response proteins expressed could inhibit further transcription initiation viral promoters. Our objective was determine effect interferon-γ(IFN-γ) on transgene driven a SV40 or CMV promoter/enhancer major histocompatibility complex class I (MHC I) gene. We transfected luciferase three promoters into 14 cell lines many tissues several species. Luciferase assays cells untreated treated with IFN-γ indicated ...
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