Expression ratio of hepatocyte nuclear factor-1 to variant hepatocyte nuclear factor-1 in differentiation of hepatocellular carcinoma and hepatoblastoma.

摘要: Abstract Background/Aims: Liver-specific protein genes have multiple cis-/trans-acting elements, but those accountable for hepatocytic differentiation are unclear. An AT-rich core sequence (AT motif) is essential as a cis-acting element the hepatic transcription. Homologous proteins hepatocyte nuclear factor-1 (HNF-1) and variant HNF-1 (vHNF-1) bind to this motif. The ratio of vHNF-1 mRNA was examined in various liver tissues with respect their differentiation. Methods competitive reverse transcriptional polymerase chain reaction employed amplify simultaneously examine expression total RNA extracted from frozen 37 patients hepatocellular carcinoma, five hepatoblastoma, 15 non-neoplastic tissues. Results: higher well-differentiated cases than poorly-differentiated undifferentiated cases, except that one hepatoblastoma displayed high ratio. Non-neoplastic had low ratios similar reason which remained unknown. However, chronic hepatitis cirrhosis also demonstrated ratios, hence degenerative changes themselves no obvious influence on such ratios. Thus, gene seemed be differentially regulated neoplastic hepatocytes. Conclusions: These results suggested correlated histological HCC hepatoblastoma.