Somatic mutations of epidermal growth factor receptor in colorectal carcinoma

摘要: Purpose: Somatic mutations of the epidermal growth factor receptor (EGFR) gene may predict sensitivity non–small cell lung carcinoma to gefitinib. However, no have been reported for colorectal carcinoma. We therefore analyzed EGFR in adenocarcinomas by combined use laser microdissection and sequencing genomic DNA. Experimental Design: examined 11 representative adenocarcinoma lines 33 clinical samples In cases, we carefully dissected only cells from frozen sections microdissection. After DNA extraction PCR, Results: None exhibited somatic mutations, but 4 tumors (12%) kinase domain. This be first report adenocarcinoma. Conclusions: Our findings suggest that a distinct minority exhibit EGFR, these susceptible gefitinib treatment.