Normal adult ramified microglia separated from other central nervous system macrophages by flow cytometric sorting. Phenotypic differences defined and direct ex vivo antigen presentation to myelin basic protein-reactive CD4+ T cells compared.

摘要: Ramified microglia in the adult central nervous system (CNS) are principal glial element up-regulating MHC class I and II expression response to inflammatory events or neuronal damage. A proportion of these cells also express constitutively normal CNS. The role as APCs for CD4+ T extravasating into CNS remains undefined. In this study, using irradiation bone marrow chimeras CD45-congenic rats, phenotype CD45lowCD11b/c+ is shown identify microglial specifically within Highly purified populations nonmicroglial but CNS-associated macrophages (CD45highCD11b/c+) have been obtained directly from CNS, by flow cytometric sorting. Morphologically, freshly isolated vs other quite distinct. Of two recovered it minority CD45highCD11b/c+ transitional macrophage population, not microglia, that effective APC experimental autoimmune encephalomyelitis-inducing myelin basic protein (MBP)-reactive cells. stimulate MBP-reactive without addition MBP culture, suggesting presentation endogenous Ag. This first study which analyzed ability with substantial cross-contamination between eliminated. heterogeneity terms function clearly demonstrated. Evidence still lacking capacity interact proliferate secrete IL-2.