A polymerase chain reaction-based assay for the rapid detection of gene amplification in human tumors.
作者： Andreas Waha , Britta Rollbrocker , Otmar D. Wiestler , Andreas von Deimling
DOI: 10.1097/00019606-199606000-00010
关键词:
摘要: A differential polymerase chain reaction (PCR) protocol was established for semiquantitative, nonradioactive detection of gene amplification using a DNA sequencer. Oncogene fragments and control sequences were simultaneously PCR-amplified fluorescent-labelled primers. Analysis the PCR products allowed quantitative assessment copy numbers in this coamplification assay. Using approach, we examined series 132 brain tumors epidermal growth factor receptor (EGFR) gene. The same set also analyzed by Southern blotting hybridization with radiolabelled EGFR probe. Both methods yielded virtually identical results. This technique has great potential screening large tumor panels oncogene amplification.
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