Effect of multiple infections with white spot syndrome virus and Vibrio anguillarum on Pacific white shrimp Litopenaeus vannamei (L.): mortality and viral replication
作者： I K Jang , G Qiao , S-K Kim
DOI: 10.1111/JFD.12194
关键词:
摘要: Multiple infections are commonly found in practical shrimp culture and may cause more serious consequences than by one pathogen only. Therefore, this study was conducted to evaluate the effect of multiple with white spot syndrome virus (WSSV) Vibrio anguillarum on Pacific Litopenaeus vannamei (L.), mortality, WSSV replication vivo host immune response. In single-infection group (WSSV load, 2 × 10(2) copies μL(-1)), mean cumulative mortality 29.2%. V. group, 12.5% when were challenged 10(5) CFU mL(-1) bacteria. co- super-infection groups, 37.5% 50% mortalities, respectively, observed at a lower bacterial concentration 10(3) mL(-1), suggesting that died earlier frequently singly infected shrimp. load after injection tracked over time TaqMan quantitative PCR. increased rapidly multiple-infection groups group. Additionally, mRNA expression genes encoding prophenoloxidase 1 2, which closely involved innate immunity shrimp, down-regulated extensively as indicated reverse-transcription
sci-hub.se 参考文章(25)
C M Escobedo-Bonilla, V Alday-Sanz, M Wille, P Sorgeloos, M B Pensaert, H J Nauwynck, A review on the morphology, molecular characterization, morphogenesis and pathogenesis of white spot syndrome virus Journal of Fish Diseases. ,vol. 31, pp. 1- 18 ,(2007) , 10.1111/J.1365-2761.2007.00877.X
L. H. Phuoc, M. Corteel, H. J. Nauwynck, M. B. Pensaert, V. Alday-Sanz, W. Van den Broeck, P. Sorgeloos, P. Bossier, Increased susceptibility of white spot syndrome virus‐infected Litopenaeus vannamei to Vibrio campbellii Environmental Microbiology. ,vol. 10, pp. 2718- 2727 ,(2008) , 10.1111/J.1462-2920.2008.01692.X
S V Durand, D V Lightner, Quantitative real time PCR for the measurement of white spot syndrome virus in shrimp Journal of Fish Diseases. ,vol. 25, pp. 381- 389 ,(2002) , 10.1046/J.1365-2761.2002.00367.X
Piti Amparyup, Walaiporn Charoensapsri, Anchalee Tassanakajon, Prophenoloxidase system and its role in shrimp immune responses against major pathogens. Fish & Shellfish Immunology. ,vol. 34, pp. 990- 1001 ,(2013) , 10.1016/J.FSI.2012.08.019
Eleonor V. Alapide-Tendencia, Lourdes A. Dureza, Isolation of Vibrio spp. from Penaeus monodon (Fabricius) with red disease syndrome Aquaculture. ,vol. 154, pp. 107- 114 ,(1997) , 10.1016/S0044-8486(97)00045-8
D.V Lightner, R.M Redman, Shrimp diseases and current diagnostic methods Aquaculture. ,vol. 164, pp. 201- 220 ,(1998) , 10.1016/S0044-8486(98)00187-2
Le Hong Phuoc, Mathias Corteel, Nguyen Cong Thanh, Hans Nauwynck, Maurice Pensaert, Victoria Alday-Sanz, Wim Van den Broeck, Patrick Sorgeloos, Peter Bossier, Effect of dose and challenge routes of Vibrio spp. on co-infection with white spot syndrome virus in Penaeus vannamei Aquaculture. ,vol. 290, pp. 61- 68 ,(2009) , 10.1016/J.AQUACULTURE.2009.02.004
In-Kwon Jang, Xian-Hong Meng, Hyung-Chul Seo, Yeong-Rok Cho, Bong-Rae Kim, Gopalakannan Ayyaru, Jong-Sheek Kim, A TaqMan real-time PCR assay for quantifying white spot syndrome virus (WSSV) infections in wild broodstock and hatchery-reared postlarvae of fleshy shrimp, Fenneropenaeus chinensis Aquaculture. ,vol. 287, pp. 40- 45 ,(2009) , 10.1016/J.AQUACULTURE.2008.10.038
Yumiao Sun, Fuhua Li, Jianhai Xiang, Analysis on the dynamic changes of the amount of WSSV in Chinese shrimp Fenneropenaeus chinensis during infection Aquaculture. ,vol. 376-379, pp. 124- 132 ,(2013) , 10.1016/J.AQUACULTURE.2012.11.014
Vitaya Thamavit, Suraphol Kongtim, Tirasak Pasharawipas, Timothy W. Flegel, Linda Nielsen, Presence of multiple viruses in non-diseased, cultivated shrimp at harvest Aquaculture. ,vol. 240, pp. 55- 68 ,(2004) , 10.1016/J.AQUACULTURE.2004.06.032