Methods for detecting and amplifying nucleic acid sequences using modified oligonucleotides having increased target specific t¿m?

摘要: The present invention concerns oligonucleotides containing one or more modified nucleotides which increase the binding affinity of to target nucleic acids having a complementary nucleotide base sequence. These hybridize sequence at faster rate than unmodified an identical Such include least 2'-O-methylribofuranosyl moiety joined nitrogenous base. Oligonucleotides can be in accordance with preferentially bind RNA targets. also methods using these and kits same.