Comparative pharmacokinetics of oxytetracycline in tilapia (Oreochromis spp.) maintained at three different salinities

摘要: Abstract Environmental factors, such as temperature, pH, and salinity of water may affect the pharmacokinetics (PK) a drug in aquatic animals most instances is ignored PK studies. This study compared profiles oxytetracycline (OTC) following single oral dosage 50 mg/kg tilapia (Oreochromis spp.) maintained three environments: freshwater (FW: 0 ppt salinity), brackish (BW: 15 ppt salinity) salt (SW: 30 ppt salinity). Water quality parameters between systems were similar except salinity. Following OTC administration, blood samples collected at 23 time points: 0.25, 0.5, 1, 2, 4, 6, 9, 12, 24 h, 8, 10, 14, 18, 22, 26, 30, 34, 38 42 days. At each sampling time, six fish from group netted, sedated with buffered MS-222, bled then euthanized. The was extracted plasma by Solid Phase Extraction (SPE) analyzed Ultra High Pressure Liquid Chromatography coupled tandem quadrupole mass spectrometer. concentration versus data for FW, BW SW subjected to analysis using non-compartment methods. Pharmacokinetics characterized rapid absorption slow excretion FW tilapia. Compared groups, elimination faster AUC0-∞ order (165 h.μg/mL) > BW (145 h.μg/mL) > SW (55.5 h.μg/mL) group. In tilapia, terminal half-life (69 h) >2 times shorter than (177 h) (155 h) groups. However, AUCs half-lives groups not significantly different. indicated that rise level increases clearance It suggested residues tissues will be same different levels. results confirmed infectious diseases associated bacteria having MIC 0.5–1.0 μg/mL can treated group, but lead therapeutic failure increased risk resistance emergence.