In vitro activity of inexpensive topical alternatives against Candida spp. isolated from the oral cavity of HIV-infected patients

摘要: Abstract The use of inexpensive topical alternatives, e.g. oil melaleuca (tea tree (TTO)), chlorhexidine (CHX), povidone iodine (PI) and gentian violet (GV), to treat oral candidiasis in human immunodeficiency virus (HIV)-infected patients has been proposed resource-poor countries. However, pre-clinical studies comparing the antifungal activity these agents are lacking. This study compared minimal inhibitory concentrations (MICs) TTO, GV, PI, CHX fluconazole (FLZ) against 91 clinical Candida strains using Clinical Laboratory Standard Institute (CLSI) methodology. Isolates were obtained from cavity acquired immune deficiency syndrome (AIDS) patients. Among examined, GV showed most potent all isolates tested (MIC range, MIC for 50% organisms (MIC50) 90% (MIC90) 0.03–0.25 μg/mL, 0.06 μg/mL 0.12 μg/mL, respectively). was 64 times less active than MIC50 MIC90 0.5–16 μg/mL, 4 μg/mL 8 μg/mL, lowest seen PI (MIC90 = 0.25%). Moreover, unlike other tested, fungicidal (minimum concentration = 1 μg/mL) albicans (n = 83). In addition, FLZ-resistant C. (n = 3). combination FLZ not antagonistic there no interaction between two compounds. possesses FLZ-susceptible -resistant is when used with FLZ. vivo evaluation warranted.