Identification of putative noncoding RNAs among the RIKEN mouse full-length cDNA collection.

摘要: With the sequencing and annotation of genomes transcriptomes several eukaryotes, importance noncoding RNA (ncRNA)-RNA molecules that are not translated to protein products-has become more evident. A subclass ncRNA transcripts encoded by highly regulated, multi-exon, transcriptional units, processed like typical protein-coding mRNAs increasingly implicated in regulation many cellular functions eukaryotes. This study describes identification candidate functional ncRNAs from among RIKEN mouse full-length cDNA collection, which contains 60,770 sequences, using a systematic computational filtering approach. We initially searched for previously reported found nine murine homologs described nonmouse ncRNAs. Through our approach filter artifact-free clones lack coding potential, we extracted 4280 as largest-candidate set. Many set had EST hits, potential CpG islands surrounding transcription start sites, homologies with human genome. implies candidates indeed transcribed regulated manner. Our results demonstrate major mammals.