Distinctive Molecular Profiles of High-Grade and Low-Grade Gliomas Based on Oligonucleotide Microarray Analysis
作者： David E. Misek , Samir M. Hanash , Rork Kuick , Mila Blaivas , Miroslav P. Bobek
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摘要: Astrocytomas are heterogeneous intracranial glial neoplasms ranging from the highly aggressive malignant glioblastoma multiforme (GBM) to indolent, low-grade pilocytic astrocytoma. We have investigated whether DNA microarrays can identify gene expression differences between high-grade and tumors. compared transcriptional profile of 45 astrocytic tumors including 21 GBMs 19 astrocytomas using oligonucleotide-based microarrays. Of ∼6800 genes that were analyzed, a set 360 provided molecular signature distinguished astrocytomas. Many transcripts increased in GBM not previously associated with gliomas found encode proteins properties suggest their involvement cell proliferation or migration. Microarray-based data for subset was validated real-time quantitative reverse transcription-PCR. Immunohistochemical analysis also localized protein products specific interest neoplastic cells Our study has identified large number novel distinct patterns gliomas.
aacrjournals.org参考文章(31)
Rodney J. Folz, Igor Nepluev, Poly(A) cDNA-specific (PACS) RT-PCR: a quantitative method for the measurement of any poly(A)-containing mRNA not affected by contaminating genomic DNA. BioTechniques. ,vol. 29, pp. 762- 768 ,(2000) , 10.2144/00294ST01
Geoffrey J. Pilkington, Abderrahim Merzak, Shahriar Koocheckpour, CD44 Mediates Human Glioma Cell Adhesion and Invasion in Vitro Cancer Research. ,vol. 54, pp. 3988- 3992 ,(1994)
E. C. Holland, Raymond E Sawaya, Gregory N Fuller, J. M. Bruner, W. K A Yung, L. S. Caskey, W. Zhang, Toward a molecular classification of the gliomas: histopathology, molecular genetics, and gene expression profiling Histology and Histopathology. ,vol. 15, pp. 971- 981 ,(2000) , 10.14670/HH-15.971
Gregory N Fuller, Chang Hun Rhee, Kenneth R Hess, Laura S Caskey, Ruoping Wang, Janet M Bruner, WK Alfred Yung, Wei Zhang, None, Reactivation of Insulin-like Growth Factor Binding Protein 2 Expression in Glioblastoma Multiforme A Revelation by Parallel Gene Expression Profiling Cancer Research. ,vol. 59, pp. 4228- 4232 ,(1999)
Christian Gisselbrecht, Philippe Gaulard, Dario C. Altieri, Hervé Dombret, Colette Adida, Gilles Salles, Eric Lepage, Josette Briere, Pierre Morel, Felix Reyes, Corinne Haioun, Jacques Diebold, Thierry J. Molina, Prognostic significance of survivin expression in diffuse large B-cell lymphomas Blood. ,vol. 96, pp. 1921- 1925 ,(2000) , 10.1182/BLOOD.V96.5.1921
P. Kleihues, Webster K Cavenee, None, Pathology and genetics of tumours of the nervous system. International Agency for Research on Cancer. ,(2000)
M. Bittner, P. Meltzer, Y. Chen, Y. Jiang, E. Seftor, M. Hendrix, M. Radmacher, R. Simon, Z. Yakhini, A. Ben-Dor, N. Sampas, E. Dougherty, E. Wang, F. Marincola, C. Gooden, J. Lueders, A. Glatfelter, P. Pollock, J. Carpten, E. Gillanders, D. Leja, K. Dietrich, C. Beaudry, M. Berens, D. Alberts, V. Sondak, N. Hayward, J. Trent, Molecular classification of cutaneous malignant melanoma by gene expression profiling Nature. ,vol. 406, pp. 536- 540 ,(2000) , 10.1038/35020115
Kai Stoeber, Alex Freeman, Anthony D. Mills, Nicholas Coleman, Lesley S. Morris, Gareth H. Williams, Ronald A. Laskey, Minichromosome maintenance proteins as biological markers of dysplasia and malignancy. Clinical Cancer Research. ,vol. 5, pp. 2121- 2132 ,(1999)
G Reifenberger, J Reifenberger, K Ichimura, V P Collins, P S Meltzer, Amplification of Multiple Genes from Chromosomal Region 12q13-14 in Human Malignant Gliomas: Preliminary Mapping of the Amplicons Shows Preferential Involvement of CDK4, SAS, and MDM2 Cancer Research. ,vol. 54, pp. 4299- 4303 ,(1994)
M. B. Sevigny, J. Ye, S. Kitazume-Kawaguchi, F. A. Troy, Developmental expression and characterization of the alpha2,8-polysialyltransferase activity in embryonic chick brain. Glycobiology. ,vol. 8, pp. 857- 867 ,(1998) , 10.1093/GLYCOB/8.9.857