Immunohistochemical study of the expression of MUC6 mucin and co-expression of other secreted mucins (MUC5AC and MUC2) in human gastric carcinomas.

作者: Celso A. Reis , Leonor David , Filipa Carvalho , Ulla Mandel , Carme de Bolós

DOI: 10.1177/002215540004800307

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摘要: To investigate the expression of MUC6 mucin in gastric carcinomas, we generated a novel monoclonal antibody (MAb CLH5) using an synthetic peptide. MAb CLH5 reacted exclusively with peptide and native deglycosylated extracts from tissues. immunoreactivity was observed normal mucosa restricted to pyloric glands antrum mucopeptic cells neck zone body region. In series 104 31 (29.8%) were immunoreactive for MUC6. The not associated histomorphological type or clinicopathological features carcinomas. Analysis co-expression other secreted mucins (MUC5AC MUC2) 20 carcinomas revealed that different core proteins are co-expressed 55% cases. co-localized MUC5AC 45% MUC2 5% Expression alone 25% All expressing more than 50% mucinous according WHO classification. independent stage tumors. conclusion, observed, well-characterized MAb, is good marker cell differentiation expressed 30% Furthermore, found co-localization histomorphology staging. Finally, intestinal as most prominent tested mucinous-type

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