Absence of cytomegalovirus in high-coverage DNA sequencing of human glioblastoma multiforme.

摘要: Cytomegalovirus (CMV) has been proposed to be associated with glioblastoma multiforme, but there are conflicting results including lack of CMV mRNA in transcriptome sequencing data. Here, we utilized deep-coverage whole-genome data detect latent DNA surgically resected tumors and assess the relative proportions viral human DNA. We did not find traces 52.6 billion reads from 34 glioblastomas. By statistical analysis, conclude that should virus present these tumors, average level does exceed one per 240,000 tumor cells (99% CI).