Genetic Manipulation of Genes and Cells in the Nervous System of the Fruit Fly

摘要: Research in the fruit fly Drosophila melanogaster has led to insights neural development, axon guidance, ion channel function, synaptic transmission, learning and memory, diurnal rhythmicity, disease that have had broad implications for neuroscience. is currently eukaryotic model organism permits most sophisticated in vivo manipulations address function of neurons neuronally expressed genes. Here, we summarize many techniques help assess role specific by labeling, removing, or altering their activity. We also survey genetic identify characterize genes mutation, overexpression, protein labeling. attempt acquaint reader with available options contexts apply these methods.