Characterization of a K+-Stimulated Adenosine Triphosphatase Associated with the Plasma Membrane of Red Beet
作者： Donald P. Briskin , Ronald J. Poole
DOI: 10.1104/PP.71.2.350
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摘要: A membrane fraction enriched with a magnesium-dependent, monovalent cation-stimulated ATPase was isolated from red beet (Beta vulgaris L.) storage roots by combination of differential centrifugation, extraction KI, and sucrose density gradient centrifugation. This distinct endoplasmic reticulum, Golgi, mitochondrial, possibly tonoplast membranes as determined an analysis marker enzymes. The activity associated this further characterized found to have pH optimum 6.5 in the presence both Mg2+ K+. substrate specific for ATP had temperature near 40°C. Kinetics Mg:ATP followed simple Michaelis-Menten relationship. However kinetics K+-stimulation were complex suggestive negative cooperativity. When cations present at 2.5 millimolarity, stimulated sequence K+ > Rb+ Na+ Li+ but when concentration raised 50 changed ≥ Li. not synergistically combinations enzyme insensitive NaN3, oligomycin, ouabain, sodium molybdate sensitive N,N′-dicyclohexylcarbodiimide, diethylstilbestrol, vanadate. Based on similarity between properties those other well plant tissues, it has been concluded that is plasma vesicles.
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