Expression of oligodendrocyte lineage genes in oligodendroglial and astrocytic gliomas.

摘要: The oligodendrocyte lineage genes OLIG1 and OLIG2 have been reported as potential diagnostic markers for oligodendrogliomas [Lu et al. (2001) Proc Natl Acad Sci USA 98:10851–10856; Marie Lancet 358:298–300]. We investigated the mRNA expression of OLIG2, well four other involved in development (E2A, HEB, NKX2.2, PDGFRA) a panel 70 gliomas, including 9 oligodendrogliomas, 11 anaplastic 5 oligoastrocytomas, 10 diffuse astrocytomas, 15 glioblastomas. Most tumors demonstrated higher transcript levels these compared to non-neoplastic adult brain tissue. Four glioblastomas showed markedly increased PDGFRA due gene amplification. Statistical analyses revealed no significant differences between oligodendroglial astrocytic tumors. In tumors, six was not significantly correlated loss heterozygosity on chromosome arms 1p 19q. Thus, is up-regulated relative tissue majority suggesting that glioma cells are arrested or recapitulate molecular phenotypes corresponding early stages glial development. However, determination by means reverse transcription-PCR does appear be diagnostically useful marker oligodendrogliomas.