The Macaque Sperm Actin Cytoskeleton Reorganizes in Response to Osmotic Stress and Contributes to Morphological Defects and Decreased Motility

摘要: Abstract Sperm undergo extreme variations in temperature and osmolality during cryopreservation, resulting cell damage that includes plasma membrane defects, changes volume, decreased motility, flagellar defects. However, the fundamental biologic mechanisms underlying these events are poorly understood. We investigated effects of osmotic stress cytochalasins b (CB) d (CD), naturally occurring toxins disrupt actin organization, on cytoskeleton motility Rhesus macaque sperm (Macaca mulatta). were diluted media low, medium, or high osmolality, medium-osmolality containing CB CD, stained with phalloidin-fluorescein isothiocyanate, processed for microscopy. The majority incubated exhibited postacrosomal stain, whereas minority displayed banding patterns F-actin stain head. High-osmolality media, as well CD incubation, resulted reorganization into bands...