Hepatic injury associated with small bowel bacterial overgrowth in rats is prevented by metronidazole and tetracycline

摘要: Susceptible rat strains develop hepatobiliary injury following the surgical creation of self-filling blind loops that cause small bowel bacterial overgrowth. Luminal bacteria or their cell wall polymers were implicated in pathogenesis lesions because sham-operated rats and with self-emptying loops, having only slightly increased counts, did not hepatic injury. In this study, antibiotics different spectra activities continuously administered starting 1 day 22 days after surgery to determine which intestinal flora may be responsible for development Four weeks surgery, Lewis receiving no had elevated liver histology scores (8.2 +/- 1.3 vs. 0.7 0.4) plasma aspartate aminotransferase levels (269 171 84 24) compared rats, P less than 0.001. Oral gentamicin as well oral intraperitoneal polymyxin B, binds endotoxin, prevent loops. However, metronidazole tetracycline therapy beginning diminished (histology score 3.0 1.8, 2.9 1.1; 87 25, 98 34; respectively 0.001 antibiotics). Metronidazole also protected Wistar require 12 experimentally induced overgrowth received antibiotic treatment 10.4 1.1, 273 239 76 20, 0.001). When started loop values decreased normal during next 77 final normal. All negative peritoneal, liver, spleen, blood cultures but approximately 75% mesenteric lymph node positive irrespective treatment. Because Bacteroides species have been causing vitamin B12 disaccharidase deficiencies we documented presence absence these organisms from using selective culture techniques. eliminated sp. B not.(ABSTRACT TRUNCATED AT 400 WORDS)