Hypobaric hypoxia-reoxygenation diminishes band 3 protein functions in human erythrocytes

摘要: We have previously shown that subjects exposed to acute hypobaric hypoxia display an erythrocyte membrane protein band 3 with increased susceptibility proteolytic degradation. suggested it was due oxidative damage of 3. now report exposure followed by reoxygenation affects functions such as anion transport and binding glyceraldehyde-3P-dehydrogenase. Transport capacity assessed the fluorescent probe 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino] ethanesulfonate (NBD-taurine). Binding evaluated from activity membrane-associated enzyme. Healthy young men were for 20 min hypoxia, simulating altitude 4,500 m above sea level after recompression function assessed. An inhibition a decrease in glyceraldehyde-3P-dehydrogenase observed. Evidence is given supporting hypothesis functional alteration its modification originated consequence further reoxygenation.