Increased expression of vascular endothelial growth factor in human hepatocellular carcinoma

摘要: Abstract Background/Aims: Angiogenesis is critical for the development and progression of solid tumors. The purpose this study was to evaluate possible role vascular endothelial growth factor (which considered be one most important factors involved in tumor-associated angiogenesis), human hepatocellular carcinoma. Methods: Vascular gene protein expression were analyzed by means Northern hybridization immunohistochemical methods 5 carcinoma cell lines. Secretion evaluated immunoblotting conditioned medium these cells. Further, we compared level tissues along with that surrounding tumor-free obtained from 20 patients. We also mRNA Flt-1, specific high-affinity receptors, Results: analysis immunohistochemistry revealed all cultured cells exhibited a high mRNA. In addition, secretion Hep G2, lines, shown Western blot. vivo , observed both non-hepatocellular tissues. However, 12 cases, levels significantly up-regulated majority cases (10 out cases) abundant tumor vascularity, up-regulation observed. failed detect Flt-1 Conclusuions: This suggest possibility overexpress protein. These findings support hypothesis angiogenesis carcinoma, may even and/or itself.