The expression of p185(HER-2/neu) correlates with the stage of disease and survival in colorectal cancer.

摘要: Abstract BACKGROUND & AIMS: HER-2/neu oncogene encodes a transmembrane tyrosine kinase receptor that is amplified and/or overexpressed predominantly in adenocarcinomas. This phenomenon has been most intensively studied breast carcinoma where its amplification and overexpression correlate with the overall course of disease poor prognosis. study was designed to investigate gene expression benign malignant colorectal lesions evaluate prognostic importance cancer. METHODS: Two hundred twenty-one samples normal colon, lesions, adenocarcinomas were for oncoprotein. Immunohistochemical staining formalin-fixed, paraffin-embedded tissue sections primary tumor lymph nodes performed. Immunoprecipitation followed by Western blotting freshly frozen same tumors also RESULTS: Normal colon mucosa, clearly differed levels histological distribution p185(HER-2/neu). mucosa mostly negative, but significant number protein. Adenocarcinomas significantly more positive than lesions. The results show correlation epithelial abnormality degree clinical parameters including Dukes' classification relapse-free postoperative survival period. CONCLUSIONS: p185(HER-2/neu) rate could serve as an independent factor patients p185(HER-2/neu)-positive malignancies. (Gastroenterology 1997 Apr;112(4):1103-13)