BEAMing up for detection and quantification of rare sequence variants
作者： Meng Li , Frank Diehl , Devin Dressman , Bert Vogelstein , Kenneth W Kinzler
DOI: 10.1038/NMETH850
关键词:
摘要: BEAMing allows the one-to-one conversion of a population DNA fragments into beads. We used rolling circle amplification to increase number copies bound such beads by more than 100-fold. This allowed enumeration mutant and wild-type sequences even when they were present at ratios less 1:10,000 was sensitive enough directly quantify error rate polymerases for PCR.
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