CD44+CD24− prostate cells are early cancer progenitor/stem cells that provide a model for patients with poor prognosis
作者： E M Hurt , B T Kawasaki , G J Klarmann , S B Thomas , W L Farrar
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摘要: Recent evidence supports the hypothesis that cancer stem cells are responsible for tumour initiation and formation. Using flow cytometry, we isolated a population of CD44+CD24− prostate display cell characteristics as well gene expression patterns predict overall survival in patients. form colonies soft agar tumours NOD/SCID mice when few 100 injected. Furthermore, express genes known to be important maintenance, such BMI-1 Oct-3/4. Moreover, can maintain stem-like nonadherent spheres serum-replacement media without substantially shifting expression. Addition serum results adherence plastic shifts resemble differentiated parental cells. Thus, propose provide genomic definition these they give rise to. have signature is predictive poor patient prognosis. Therefore, LNCaP offer an attractive model system both explore biology maintenance differentiation develop therapeutics, pattern consistent with
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