Fermented ginseng extract, BST204, disturbs adipogenesis of mesenchymal stem cells through inhibition of S6 kinase 1 signaling.

摘要: Abstract Background The biological and pharmacological effects of BST204, a fermented ginseng extract, have been reported in various disease conditions. However, its molecular action metabolic remains poorly understood. In this study, we identified the antiadipogenic activity BST204 resulting from inhibition S6 kinase 1 (S6K1) signaling pathway. Methods inhibitory on S6K1 were investigated by immunoblot, nuclear fractionation, immunoprecipitation analyses. effect was evaluated measuring mRNA levels adipogenic genes chromatin quantitative real-time polymerase chain reaction analysis. Results Treatment with inhibited activation translocation S6K1, further decreasing interaction between histone H2B 10T1/2 mesenchymal stem cells. Subsequently, phosphorylation at serine 36 (H2BS36p) reduced inducing an increase expression Wnt6, Wnt10a, Wnt10b, which disturbed differentiation promoted myogenic early osteogenic gene expression. Consistently, treatment during commitment suppressed marker lipid drop formation. Conclusion Our results indicate that blocks adipogenesis cells through S6K1-mediated phosphorylation. This study suggests potential therapeutic strategy using to combat obesity musculoskeletal diseases.