Influence of mitochondria on gene expression in a citrus cybrid

摘要: The production of cybrids, combining nucleus a species with alien cytoplasmic organelles, is valuable method used for improvement various crops. Several citrus cybrids have been created by somatic hybridization. These genotypes are interesting models to analyze the impact genome change on nuclear expression. Herein, we report genome-wide gene expression analysis in leaves cybrid between C. reticulata cv ‘Willowleaf mandarin’ and limon ‘Eureka lemon’ compared its lemon parent, using Citrus 20K cDNA microarray. Molecular showed that this possesses chloroplast genomes Eureka plus mitochondria from Willowleaf mandarin and, therefore, can be considered as bearing foreign mitochondria. Mandarin influenced large set genes causing an over-expression 480 them repression 39 genes. Quantitative real-time RT–PCR further confirmed credibility microarray data. Genes over-expressed predominantly attributed functional category “cellular protein metabolism” whereas down-regulated none was enriched. Overall, replacement affected different including particularly predicted involved mitochondrial retrograde signaling. Mitochondria regulate all cell structures even status. results suggest modulated respect new information received organelle, final objective suit specific needs ensure better physiological balance.