The intermediate filament protein nestin occurs transiently in differentiating testis of rat and mouse

摘要: Nestin is an intermediate filament (IF) protein (IFP) which occurs during early developmental stages and regenerative processes in muscle neuronal cells. The spatial temporal localization of nestin the developing testis rat mouse was studied by immunolabeling light electron microscopy immunoblotting. related to other major IFPs specific for this tissue, i.e. cytokeratins, vimentin desmin. Laminin immunocytochemistry conventional were used identify tissues With incipient differentiation gonadal anlage, reaction weak ridge, whereas cells mesonephric mesenchyme showed a prominent IFP. nestin-specific epithelial duct tubules disappeared at phase differentiation. development proper, transiently found several cell types. as well cytokeratins Sertoli In interstitial together with desmin IFPs, most differentiating myoid After birth, gradually from testicular puberty only endothelial some blood vessels. abolished synthesis confirmed These results suggest that required mesonephros. temporary presence nestin, these phases, coincides key phases urogenital sex This may imply orchestrated likely be linked genes regulating