Plant Tissues as 3D Natural Scaffolds for Adipose, Bone and Tendon Tissue Regeneration.

摘要: Decellularized tissues are a valid alternative as tissue engineering scaffolds, thanks to the three-dimensional structure that mimics native be regenerated and biomimetic microenvironment for cells growth. Despite decellularized animal have long been used, plant scaffolds might overcome availability issues, high costs ethical concerns related use of sources. The wide range features covered by different plants offers unique opportunity development tissue-specific depending on morphological, physical mechanical peculiarities each plant. Herein, three (i.e., apple, carrot, celery) were and, according their peculiar properties porosity, properties), addressed regeneration adipose tissue, bone tendons, respectively. carrot celery maintained porous structure, with pores ranging from 70 420 μm, source, stable in PBS at 37°C up 7 weeks. Different Eapple = 4 kPa, Ecarrot 43 Ecelery 590 kPa) measured no indirect cytotoxic effects demonstrated vitro after decellularization. After coating poly-L-lysine, apples supported 3T3-L1 preadipocytes adhesion, proliferation adipogenic differentiation; carrots MC3T3-E1 pre-osteoblasts osteogenic L929 guided anisotropic orientation. versatile potential proved this work.