Molecular characterization of Citrus tatter leaf virus historically associated with Meyer lemon trees: complete genome sequence and development of biologically active in vitro transcripts

摘要: Tatineni, S., Afunian, M. R., Hilf, E., Gowda, Dawson, W. O., and Garnsey, S. 2009. Molecular characterization of Citrus tatter leaf virus historically associated with Meyer lemon trees: Complete genome sequence development biologically active in vitro transcripts. Phytopathology 99:423-431. isolated from trees (CTLV-ML) California Florida induces bud union incompatibility citrus grafted on the widely used trifoliate hybrid rootstocks. The complete CTLV-ML was determined to be 6,495 nucleotides (nts), two overlapping open reading frames (ORFs) a poly (A) tail at 3′ end. organization is similar other capilloviruses, ORF1 (nts 37 6,354) encoding putative 242-kDa polyprotein which contains replication-associated domains plus coat protein (CP), ORF2 4,788 5,750), located within different frame encodes movement protein. Although proteins encoded by possesses 84 96% amino acid identity strains Apple stem grooving (ASGV), we observed strikingly regions ORF1: variable region I (amino acids 532 570) II 1,583 1,868), only 15 18 56 62% identities, respectively, corresponding ASGV strains. Conditions for herbaceous systemic assay host were optimized wildtype induced infection Phaseolus vulgaris cv. Light Red Kidney (LRK) bean plants 19 or 22°C but not higher temperatures. In transcripts generated full-length cDNA clones symptoms LRK that wild-type virus. Replication recombinant confirmed hybridization 5′ positive-stranded RNA-specific probe genome-sized RNA reverse-transcription polymerase chain reaction.