Different NK Cell Surface Phenotypes Defined by the DX9 Antibody Are Due to KIR3DL1 Gene Polymorphism

摘要: KIR3DL1 and KIR3DL2 are NK cell receptors for polymorphic HLA-B -A determinants. The proportion of cells that bind anti-KIR3DL1-specific Ab DX9 their level binding vary between individuals. To determine whether these differences due to KIR polymorphism, we assessed KIR3D gene diversity in unrelated individuals families. Both highly genes, with KIR3DS1 segregating like an allele KIR3DL1. A haplotype lacking was defined. two alleles a heterozygous donor were expressed by different, but overlapping, subsets clones. Sequence variation appear distinct; recombination is more evident KIR3DL1, point mutation KIR3DL2. genotype correlates well levels cells, not the frequency DX9-binding cells. Different high, low, no Ab. Consequently, heterozygotes high low have distinct subpopulations at levels, giving characteristic bimodal distributions flow cytometry. Z27 gave patterns similar those DX9. Four producing phenotypes distinguished from four or substitution one positions encoded protein: 182 283 extracellular Ig-like domains, 320 transmembrane region, 373 cytoplasmic tail.