Biocompatibility testing of a bovine hydroxy—apatite ceramic material with the use of osteo-progenitor cells isolated from human bone marrow

摘要: Bioactive materials such as hydroxyapatite ceramics are known to show a stable interfacial bond with tissues. The objective of this study was investigate the behaviour human bone differentiated cells isolated from marrow on surface ceramic obtained bovine spongiosa. Scanning electron microscopy and DNA synthesis analysis assessed by [3H]-thymidine incorporation showed cell colonization whole material. Immunological studies using monoclonal antibodies osteocalcin osteonectin cytochemical alkaline phosphatase activity indicated that these did not lose their osteoblastic phenotype after 28 days culture. Furthermore, demonstrates in vitro interface between material cells, which is reporduced vivo.