Physical and functional interactions between p53 and cell cycle co-operating transcription factors, E2F1 and DP1.

摘要: One way in which wild-type p53 is able to regulate cell cycle progression thought be via the induction of its downstream target gene Waf1/CIP1, thus indirectly regulating transcriptional activity E2F. The E2F transcription factors are known key effectors cycle. We report here that there a physical and functional interaction between two components factors, E2F1 DP1. expression can inhibit E2F, both DP1 also downregulate p53-dependent transcription. inhibited by direct binding mdm2, but we demonstrate independently mdm2. Detailed studies protein-protein interactions have provided evidence co-operating factor complex with vitro vivo.