Citrate metabolism in red blood cells stored in additive solution-3.

摘要: BACKGROUND Red blood cells (RBCs) are thought to have a relatively simple metabolic network compared other human cell types. Recent proteomics reports challenge the notion that RBCs mere hemoglobin carriers with limited activity. Expanding our understanding of RBC metabolism has key implications in many biomedical areas, including transfusion medicine. STUDY DESIGN AND METHODS In-gel digestion coupled mass spectrometric analysis approaches were combined state-of-the-art tracing experiments by incubating leukofiltered additive solution-3 for up 42 days under bank conditions, presence 13C1,2,3-glucose, 2,2,4,4-d-citrate, and 13C,15N-glutamine. RESULTS Results indicate pentose phosphate pathway/glycolysis ratio increases during storage solution-3. While majority supernatant glucose is consumed fuel glycolysis, incorporation glucose-derived moieties was observed nucleoside monophosphates. Incubation deuterated citrate indicated uptake contribute explain origin approximately 20% 30% lactate could not be explained oxidation 2,3-diphosphoglycerate consumption alone. 13C,15N-glutamine showed glutaminolysis fuels transamination reactions accumulation millimolar levels 5-oxoproline, while de novo glutathione synthesis significantly active refrigerated storage. CONCLUSION Quantitative revealed mature can metabolize substrates than glucose, such as citrate, an observation relevant medicine (i.e., formulation novel additives), research endeavors where modulation opens potential avenues therapeutic interventions, sickle disease.