Anaplastic lymphoma kinase immunoreactivity correlates with ALK gene rearrangement and transcriptional up-regulation in non–small cell lung carcinomas
作者： Jennifer M. Boland , Sibel Erdogan , George Vasmatzis , Ping Yang , Lori S. Tillmans
DOI: 10.1016/J.HUMPATH.2009.01.012
关键词:
摘要: Recently, the fusion gene EML4-ALK was identified in non-small cell lung carcinoma, which could be a potential therapeutic target. We investigated prevalence of anaplastic lymphoma kinase protein expression these tumors by immunohistochemistry and correlated results with data from ALK molecular studies. Gene profiling performed on 35 adenocarcinomas to identify cases up-regulation, overexpression immunohistochemistry. Immunohistochemistry also an independent cohort consisting 150 squamous carcinomas evaluate utility immunostaining as screening tool. Florescence situ hybridization for locus reverse transcriptase-polymerase chain reaction were positive Transcriptional up-regulation 2 (6%) profiling. These immunohistochemistry, whereas remaining 33 completely negative. In cohort, 1 3 adenocarcinomas. The 6 showed evidence rearrangement florescence but negative EGFR KRAS mutation. presence transcript confirmed reaction. conclusion, immunoreactivity associated transcriptional rearrangement, transcript. Anaplastic may have tool or surrogate marker techniques detect tumors.
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