Patterns of DNA Mutations and ALK Rearrangement in Resected Node Negative Lung Adenocarcinoma

摘要: Background Many studies have examined specific mutations in patients with resected lung adenocarcinoma across heterogeneous stages, comprising predominantly advanced/metastatic disease, but there is little data regarding the mutation profile of early stage node negative disease. The aim this study was to identify patterns adenocarcinoma. Methods A total 204 who underwent resection for IB (sixth Ed American Joint Committee on Cancer) and received no neoadjuvant or adjuvant treatments were identified. Tumors genotyped using OncoCarta v1.0 kit (Sequenom, San Diego, CA) Sequenom MassARRAY platform. Fluorescence situ hybridization ALK rearrangement also performed. Results 110 (54%) patients' tumors harbored at least one mutation. KRAS , EGFR PIK3CA PDGFRA AKT1 BRAF FGFR1 HRAS detected from 77 (37.7%), 29 (14.2%), 9 (4.4%), 2 (1%), 1 (0.5%), (0.5%) respectively. Synchronous (either comutations double mutations) identified 18 (8.8%) patients. associated poorly differentiated ( p = 0.03; 0.02), whereas well-differentiated 0.001). Five tumours contained (one T790M four exon 20 insertions), which are resistance tyrosine kinase inhibitors (EGFR-TKIs). Conclusions Diverse seen node-negative including an unexpectedly low rate rearrangement, EGFR-TKIs a high synchronous mutations. These may influence design future targeted therapy trials.