Growth factors in gliomas: antisense and dominant negative mutant strategies.

摘要: Antisense and dominant negative mutant strategies were developedas 'magic bullets' to suppress the function ofa particular gene while preserving remaining cellularactivities. While experience with these techniques has dispelledsome of 'magic', remain usefulfor understanding products.Antisense involve administration either asynthetic oligodeoxy-nucleotide or a plasmid construct which producesa sequence that is complementary DNAor mRNA interest. Antisensebinding should inhibit transcription translation thegene, thus decrease synthesis proteinfor encodes. Conversely, negativemutations activity product byencoding for second protein suppresses thefunction For example,a single subunit in multimeric proteinmight allow normal assembly whileinhibiting its activity. The use techniquesfor investigating role various growth factorpathways glial neoplasia their potential therapeuticapplications are reviewed below.