Microglial/macrophage expression of interleukin 10 in human glioblastomas.
作者： Sven Wagner , Stefanie Czub , Martina Greif , Giles Hamilton Vince , Nicole S�ss
DOI: 10.1002/(SICI)1097-0215(19990702)82:1<12::AID-IJC3>3.0.CO;2-O
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摘要: Interleukin 10 (IL-10) expression has been found to be correlated with the extent of malignancy in gliomas. In vitro, IL-10 increases proliferation and migratory capacity human glioma cell lines. this study, we localized site synthesis gliomas cells microglial origin. Biopsy specimens from 11 patients malignant were processed on native tissues at early culture passages (0-4). mRNA was analyzed by RT-PCR situ hybridization. Protein quantitatively assessed ELISA supernatants, expressing determined a combination immunohistochemistry for CD68 (specific microglia/macrophage lineage) decreased passage 0 4 all samples undetectable beyond 5. Such downregulation leads steep decrease protein supernatants (below detection level, 0.05 ng/ml, 1). The hybridization immunostaining revealed that only lineage produced mRNA. Our results identify as major source which decreases markedly during primary cultures due progressive reduction microglia/macrophages present.
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